Gene description for degP
Gene name	periplasmic serine endoprotease DegP
Gene symbol	degP
Other names/aliases	ECK0160 htrA ptd
Species	Escherichia coli str. K-12 substr. MG1655
Experiment description of studies that identified degP in Escherichia coli
1 Experiment ID 373 Identified molecule protein Extracellular vesicle type Membrane vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 17787032    Organism Escherichia coli Experiment description Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli. Authors Lee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS. Journal name Proteomics Publication year 2007 Sample Escherichia coli Sample name Normal-Escherichia coli DH5α Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
2 Experiment ID 2245 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 7 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
3 Experiment ID 2247 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 - Fraction 9 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 2251 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 2252 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 2253 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 2254 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 2255 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 36648227    Organism Escherichia coli Experiment description Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes Authors Imamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M. Journal name mBio Publication year 2023 Sample Escherichia coli Sample name E. coli strain K-12 BW25113 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 2219 Identified molecule Protein Extracellular vesicle type Outer membrane vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 32854612    Organism Escherichia coli Experiment description Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles enhance immunomodulation and antimicrobial activity in RAW264.7 macrophages Authors Hu R, Lin H, Li J, Zhao Y, Wang M, Sun X, Min Y, Gao Y, Yang M. Journal name BMC Microbiol Publication year 2020 Sample Escherichia coli Sample name E. coli strain Nissle 1917 Isolation/purification methods Centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.127-1.175 g/mL, 1.271-1.295 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -